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KMID : 0881720210360020188
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Journal of Food Hygiene and Safety
2021 Volume.36 No. 2 p.188 ~ p.195
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Viable Cell Counts and Identification of Species Used for Commercial Fermented Milk Products
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Kang Tae-Sun
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Abstract
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In this study, viable cell counts and species used for fermented milk products (n=20) sold on theSouth Korean market were analyzed and compared with their label claims. In the case of viable cell counts, all productsshowed 2.7×107-5.6×1010 CFU/mL, which satisfied the standard requirements (over 1.0×107-1.0×108 CFU/mL)suggested by the Food Code of South Korea. Sequences of 16S ribosomal RNA gene amplified from pure cultures foreach product were analyzed and compared with the NCBI GenBank database. Identified species were Lactobacillusparacasei, L. acidophilus, L. plantarum, L. rhamnosus, L. helveticus, L. delbrueckii subsp. bulgaricus, Lactococcuslactis, and Streptococcus thermophilus, all of which have been approved as probiotic microorganisms in South Korea.
The frequency in use of these lactic acid bacteria was as follows: L. paracasei (n=7), L. acidophilus (n=5), L. helveticus(n=5), S. thermophilus (n=3), L. rhamnosus (n=2), L. delbrueckii subsp. bulgaricus (n=2), L. plantarum (n=1), andLc. lactis (n=1). Therefore, labeling information declaring probiotic species used should contribute to the improvementof consumer rights and trust.
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KEYWORD
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Probiotics, Lactic Acid Bacteria, 16S Ribosomal RAN, Yogurt
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